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vacuum concentrator protocol method

Centrifugal Vacuum Concentrators and Cold TrapsCentriVap Vacuum Concentrators use a combination of centrifugal force, vacuum and heat to speed evaporation of multiple small samples in applications such as molecular biology, proteomics, genomics, cell biology, microbiology, biochemistry, drug discovery and analytical chemistry. Labconco offersFile Size 2MBVACUUM CONCENTRATOR AND METHOD FOR VACUUMMethod for the vacuum concentration of samples, in which at least one vessel with a sample to be dried is arranged in a centrifuge rotor in a vacuum chamber and the vacuum chamber is sealed in an airtight manner, the centrifuge rotor is rotated, the vacuum chamber is evacuated, the at least one sample is tempered in the vacuum chamber, the pressures

PROTOCOL Extraction and determination of glycine

mL) using a vacuum concentrator system and resuspend the samples in a minimal volume of MilliQ grade water (100 200 μL). Check the effectiveness of resuspension by vortexing or pipetting up and

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Thermo Fisher releases firstever line of vacuum

Oct 02, · Chemists, chromatographers, and molecular biologists can now take advantage of the firstever line of vacuum concentrators offering a library of preprogrammed protocols, while also allowing users to create custom programs, for fast and reliable evaporation of a broad range of solvents.

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DNA methylation analyis using restriction enzyme digestion

Inactivate the enzymes by heating (look in the data sheet of the manufacturer for inactivation protocol). Reduce the sample volume to about 2030 µl in the vacuum concentrator. Do NOT dry the DNA you will not be able to dissolve it again! Add required volume of loading buffer and load the samples onto the 1.2% agarose/TAE gel.

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CTAB Protocol for the Isolation of DNA from Plant Tissues

Centrifugal Vacuum Concentrator (e.g., SpeedVac) TE Buffer (10 mM Tris, pH 8, 1 mM EDTA) Phenol/Chloroform/Isoamyl Alcohol (25241 ratio) stored under TE buffer, pH 8; Method. Plant samples can be prepared by cryogenically grinding tissue in a mortar and pestle after chilling in liquid nitrogen. Freeze dried plants can be ground at room

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US9120050B2 Product gas concentrator utilizing vacuum

US9120050B2 US13/790,473 US201313790473A US9120050B2 US 9120050 B2 US9120050 B2 US 9120050B2 US 201313790473 A US201313790473 A US

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Lipid Extraction Kit (Chloroform Free), Trial Size

14. Leave the pooled organic layer tube open and dry in a vacuum concentrator or in a dry 37ºC incubator overnight (or until dry). Note Recovery volume should be approximately 1600 µL. For faster dry down, split samples into two equal parts. 15. Resuspend the lipid extract in an organic solvent such as butanol or cyclohexane.

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VACUUM CONCENTRATOR AND METHOD FOR VACUUM

Method for the vacuum concentration of samples, in which at least one vessel with a sample to be dried is arranged in a centrifuge rotor in a vacuum chamber and the vacuum chamber is sealed in an airtight manner, the centrifuge rotor is rotated, the vacuum chamber is evacuated, the at least one sample is tempered in the vacuum chamber, the pressures prevailing in the vacuum chamber is detected, by means of the detected pressures, it is detected when the end point of the vacuum concentration

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Centrifugal Vacuum Concentrators Labconco

CentriVap Centrifugal Vacuum Concentrators use a combination of centrifugal force, vacuum and heat to speed laboratory evaporation of multiple small samples. CentriVap C Traps protect your vacuum pump from corrosive chemicals.

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Concentrator at Thomas Scientific

Allinone vacuum concentrator. DNA Clean Concentrator provides a hasslefree method for the rapid purification and concentration of high quality DNA from any step in a standard ChIP protocol. This includes samples that have undergone reverse crosslinking, Proteinase K or RNase A

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Savant SpeedVac Medium Capacity Vacuum Concentrators

Savant SpeedVac medium capacity vacuum concentrators evaporate strong acids, bases, and aggressive organic solvents. Approved for combinatorial chemistry, these concentrators incorporate an intuitive programmable user display for enhanced usability.The concentrators assist with the helpful sample preparation for easier molecular biology and organic chemistry analyses.

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INSTRUCTION MANUAL

Concentrator5. Marker PCR Original PCR300 200 DCC5procedure. Pure and Reliable Recovery with the DCC5. Shown here is the recovery of 1 µg of 100 bp marker DNA eluted into 10 µl of water analyzed using a NanoDrop® spectrophotometer. The DNA Clean Concentrator5 consistently recovers > 90% of input DNA.

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Chemiluminescent DNA Abasic (AP) Site Assay

Nov 15, 2011· 11. Decant supernatant and wick dry on paper towel. Dry DNA in vacuum concentrator, low heat for 5 min, or in a 37 C incubator until liquid has evaporated (~20 min). 12. Resuspend DNA in 50100 µL TE and store at 4 C for at least 4 h (overnight).

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Ch oosing the best vacuum concentrator for your laboratory

Ch oosing the best vacuum concentrator for your laboratory Tips and best practices from industry pioneers. Centrifugal vacuum concentration is a unique method used for removing solvents from samples to concentrate or dry organic solvent vapors to protect vacuum pumps. Change your sample protocol

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Ch oosing the best vacuum concentrator for your laboratory

vacuum concentrator, trap, rotor and pump, fully ready to install and run upon arrival Use the charts below to select the right system for your application. Email [email protected] for any ordering questions or to discuss a customized system to suit your needs. Small and medium capacity vacuum concentrator kits by model

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Desalting/Buffer Exchange and Concentration for Affinity

PD MultiTrap G25 96well plates are designed for highthroughput desalting, buffer exchange, and cleanup of proteins, with high reproducibility welltowell and platetoplate (Figure 11.4).Using the 96well plates, multiple samples can be run conveniently and reproducibly in parallel (Figure 11.5).PD MultiTrap G25 can be operated manually or in automated mode using a robotic system

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Understanding Concentration and Evaporation Technology

Feb 06, 2013· Applying vacuum in a system decreases a solvents boiling point so that liquid vaporization occurs at lower temperatures that are safe for the sample. Heat and temperature are related by the equation Q = m cΔ T , where Q is heat energy added, m is the mass of the object, c is the specific heat capacity of the heated object, and Δ T is the change in temperature.

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Lipid Extraction Kit (Chloroform Free) Cell Biolabs

14. Leave the pooled organic layer tube open and dry in a vacuum concentrator or in a dry 37ºC incubator overnight (or until dry). Note Recovery volume should be approximately 1600 µL. For faster dry down, split samples into two equal parts. 15. Resuspend the lipid extract in an organic solvent such as butanol or cyclohexane.

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NMR Spectroscopy of Large Functional Current Protocols

Centrifugal vacuum concentrator (Vacuum concentrator plus; Eppendorf) NOTE This protocol starts with the purification of a previously transcribed RNA. For the in vitro transcription, refer to Basic Protocol 1, steps 115. 1. Work RNase free. 2. Transcribe RNA as described in Basic Protocol 1, steps 115. 3.

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Highthroughput and highsensitivity Nature Protocols

Sep 06, · In a correctly working Eppendorf Vacuum Concentrator Plus at Step 21, ~10 µl of sample should remain after 30 min of concentration at 45 °C using the VAQ program.

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Vacuum Concentrators Fisher Scientific

Thermo Scientific SpeedVac Modular Vacuum Concentrators are designed to evaporate strong acids, bases and aggressive organic solvents. Approved for combinatorial chemistry, these concentrators incorporate an intuitive programmable user display for

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The Isolation of Stress Granules From Current Protocols

Sep 18, · Vacuum concentrator (e.g., SpeedVac) Vacuum pump; 1. To beads, add 1 ml precooled (20°C) extraction buffer 1 and vortex vigorously. 2. Incubate for 10 min at 4°C with shaking. 3. Perform a short, 10min sonication treatment by partially submerging tubes in an ultrasonic bath to ensure metabolite extraction from the beads. 4.

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Thermo Scientific Savant DNA SpeedVac Concentrators

Concentrator Volume 0.4 100 mL 0.5 2.0 mL Concentrator Capacity 4 120 tubes 36 72 tubes 2 shallowwell microplates 2 shallowwell microplates Vacuum Pump ETFE oilfree diaphragm pump ETFE oilfree diaphragm pump Capacity 36 L/min at 60 Hz 36 L/min at 60 Hz 30 L/min at 50 Hz 30 L/min at 50 Hz Vacuum Control

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Utilization of Agilent SureSelect Target Enrichment for

concentrated using a vacuum concentrator to the volume stated in the protocol (3.4 µL for standard 200 ng/3 µg SureSelectXT and 12 µL for SureSelectXT HS/SureSelectXT low input). If concentrating the sample, avoid total dehydration of prehybridization libraries where possible, although this Figure 2.

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Mass SpectrometryBased Absolute Quantification of Single

Pipette tips (Rainin P200) and equipment for preparation of StageTips (see Steps 1114 of Protocol Enrichment of Phosphopeptides via Immobilized Metal Affinity Chromatography [Swaney and Villen ]) Speed vacuum concentrator. Thermoshaker (Eppendorf) at 20°C. Tubes (0.2 and 1.5mL)

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A protocol for the gentle purification of viruslike

Dec 01, · This is overcome by concentration of the sample on the vacuum concentrator. Other methods such as centrifugal filter concentrators may be used but often require pure starting material in order to avoid clogging the filter unit during centrifugation. Moreover, some VLPs may adsorb onto the filters, thereby reducing recovery.

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Concentrating heat sensitive samples without degradation

Jul 02, 2013· Some vacuum concentrators heat the chamber as high as 38˚ C, even if the temperature set point is ambient. By using a refrigerated vacuum concentrator, set point can be as low as 4˚ C to prevent the chamber from warming up. During concentration, evaporative cooling keeps the samples c at the front end of the evaporation step.

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RNAstable® Protocol SigmaAldrich

RNAstable is a unique storage medium that preserves RNA samples at room temperature. RNAstable enables you to store RNA samples for months. Using RNAstable, you can completely recover your RNA samples by simple rehydration just add water. Rehydrated samples are ready for immediate use, without the need for further purification.

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LCTech GmbH

with extraction protocol, chromatograms and recovery rates. We help you to implement the new method with suitable accessories. Read more New video Vacuum Manif EluVac DEVA Vacuum Concentrator DEVA Automated EVAporation of PCDD/F Extracts to 100 200 Microliter

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K21650 Lipid Extraction Kit (Chloroform Free)

Vacuum Concentrator Dounce homogenizer ( # 19981) Vortex VI. Storage and Reagents Preparation Store kit at RT, protected from light. Read entire protocol before performing the assay. Stable for one . VII. Lipid Extraction Protocol 1. Sample Preparation

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Savant SpeedVac Concentrators

Thermo Scientific has been manufacturing centrifugal vacuum concentrators for well over 30 s. As market leader, Thermo Scientific is able to offer a diverse product range providing solutions for a wide variety of solvent applications. These range from the traditional drying down of DNA

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